Endocrine Abstracts

Androgens are sex steroid hormones fundamental for human reproduction. In males, they are produced upon luteinizing hormone (LH) action through its specific receptor (LHCGR) expressed in Leydig cells, supporting spermatogenesis. The human chorionic gonadotropin (hCG), acting on the same receptor but not expressed in men, is administered to improve testosterone synthesis in specific pathological contexts, such as hypogonadotropic hypogonadism and cryptorchidism. In vitro</e...

Objective: The aim of this study was to identify changes in thyroid hormone metabolism occurring in different thyroid functional states and to evaluate tissue response to thyroid hormone changes.Methods: Two hundred subjects (137 F, 63 M, mean age: 50.3±14.4 years) waiting to be treated or treated with total thyroidectomy for differentiated thyroid cancer were enrolled in this study. Thyroid function was investigated measuring TSH, fT4 and fT3, and ...

During the menstrual cycle, selection of the dominant follicle is characterized by changes of gonadotropin and steroid hormone receptor expression. We aim to evaluate whether co-existing follicle-stimulating hormone receptor (FSHR) and G protein-coupled estrogen receptor (GPER) form heterodimers, suggesting their role in ovarian follicle selection. FSHR-GPER dimerization was evaluated in transfected HEK293 cells in vitro by bioluminescence resonance energy transfer (B...

Sphingosine-1 phosphate (S1P) is a lysosphingolipid present in the ovarian follicular fluid at the nanomolar concentration. The physiological functions of S1P are mediated through five specific G protein-coupled receptors (GPCRs), known as S1PR1-5. S1PR1 and S1PR3 are expressed in human primary granulosa lutein cells (hGLC), as well as in the immortalized human primary granulosa cell line hGL5. S1PRs are activated at nanomolar concentrations inducing specific activation or inh...

Background: The evaluation of health-related Quality of Life (QoL) is becoming a key component in clinical assessment of thyreopathic patients, especially those thyroidectomized for differentiated thyroid cancer (DTC) and treated with levothyroxine (LT4).Aim: To evaluate QoL in different thyroid states in patients treated with total thyroidectomy for DTC and correlate it with thyroid function tests.Methods: Two hundred subjects (13...

The concurrence of glucose intolerance, hypertension, dyslipidemia and obesity has been termed metabolic syndrome. Each component of the metabolic syndrome is an independent risk factor for cardiovascular disease and diabetes type 2, the combination of these risk factors elevating the rates and severity of cardiac pathology.The therapeutic use of irbesartan, a selective blocker of the AT1 receptors, administered in arterial hypertension, coronary heart d...

Introduction: Adequate glycaemic, along with other cardiovascular risk factor control in type 2 Diabetes (T2D), and its beneficial impact on subclinical inflammation is associated with better microvascular outcomes.Objectives: To analyse the correlation between glycaemic control adequacy and systemic inflammatory markers, renal disease progression and cardiovascular risk factors.Material and methods: Retrospective cohort observatio...

Objective: Thyroid fine-needle aspiration (FNA) cytology is indeterminate in 15–25% of cases. Recently, cytological analysis was combined to molecular analysis to improve diagnostic accuracy. In the present study, washing liquid of FNA (wFNA) samples were tested for the BRAF V600E mutation, using high resolution melting (HRM) technology. The aim of this study was to demonstrate whether BRAF mutation analysis is accurate in wFNA and, when combined with cytological analysis...

Introduction: FSH acts on its receptor (FSHR) resulting in signal transduction activation, gene expression and steroidogenesis. The FSHR common SNP p.N680S is a marker of gonadal response in vivo. However, in vitro dose–response experiments failed to demonstrate the molecular basis thereof so far. In this study, we systematically investigated whether p.N680S mediates different kinetics of FSH response in vitro.Des...

Experimental autoimmune thyroiditis (EAT) has been used to simulate human autoimmune thyroid disease for decades. EAT can be easily induced in genetically susceptible strains of mice by excess iodine ingestion or by immunization with mouse thyroglobulin. Excess iodine may induce and exacerbate autoimmune thyroiditis (AIT) in humans and animal models. In order to assess the potential protective mechanisms of selenium (Se) in thyroid autoimmunity, we evaluated the effects of ino...